Sequence assemblies obtained by DBG and OLC approaches were quality assessed using standard assembly metrics such as N50, total number of contigs, and total length of the assembly (supplementary file S2, table S1, Supplementary Material online).
We identified four novel polymorphisms (one single nucleotide deletion and three SNPs) that were not previously reported to the SNP databases among the total of 19 polymorphisms (Supplementary Table 2, see section on supplementary data given at the end of this article).
A total of 18% (141) of the supplementary orthologs, detected originally by GreenPhyl, were identified by Inparanoid using a 30% threshold.
The C. oliveri cp genome encodes a total of 113 genes (supplementary table S1, Supplementary Material online), including 4 ribosomal RNA genes, 27 transfer RNA genes, and 82 protein genes.
We inferred a total of 3,434 clusters (supplementary table S1, Supplementary Material online) that, on average, are conserved in 5.9 Drosophila species and encompass 6.4 genes (fig. 1).
To refine the phylogeny of subhaplogroup E-M35, and to obtain new insights into its geographic distribution, a total of 62 mutations (supplementary table S2, Supplementary Material online) were hierarchically genotyped in 5,222 samples belonging to 118 populations.
An incubation time of 1 h was chosen rather than manufacturer-recommended 15 min, as 2-DG slightly decreased the rate of product (NADH) formation, but did not affect the final (total) amount of the product detected (Supplementary Figure 1).
We identified 22 randomized trials that satisfied the inclusion criteria and enrolled a total of 22 434 patients (supplementary fig 1).
This portion of the genome is distributed across 198 segments carrying a total of 311 genes (Supplementary Note 4, Table S6 and Fig. S11).
A total of 47 SNPs (Supplementary Material, Table S3) were carried forward to the replication cohorts, which contained 2286 cases and 3160 controls derived from Ireland, Scotland, Sweden and Germany.
One could argue that M2 was compensating for the absence of M1 – however, the large day-to-day fluctuations in the total amount of guarding (Supplementary material 2) are not compatible with the notion that there is a certain daily "quota" of guarding.
