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Fruit was considered as the total of reads generated from pre-climacteric, climacteric and post-climacteric libraries.
To establish a threshold for this criterion, we determined the percentage of reads corresponding the most abundant unique sequence respect to the total of reads spanning the junction.
It is important to notice that this procedure conserve the amount of statistical evidence existent in the original matrix, given that the transformed matrices have the same total of reads (sum per column) than the original matrix.
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Second, misaligned reads represent a fraction of the total amount of reads generated and aligned.
The total amount of reads were 512,236 paired reads and 112, 079 single reads.
australiensis Total number of reads: 135,769; Total reads are repetitive: 86.35%; Reads that are 70 100% repetitive: 73.06%.
brachyantha Total number of reads: 67,364; Total reads are repetitive: 66.06%; Reads that are 70 100% repetitive: 26.79% (GIF 2 kb) Fig S1 aO.
Genome coverage as dictated by the total number of reads for enriched and total input fractions.
A rarefaction curve, of the total number of reads obtained vs the total number of miRNA species, was plotted.
Subsequently, counts were normalized by making sample-wise total numbers of reads equal to the median total number of reads for all samples.
We replace the original total number of reads for library with the 'effective' total number of reads.
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