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Circles represent the total of proteins identified in proteome of C. necator H16 grown in REG glycerol bottom (REG-GB), REG free fatty acid (REG-FFA) and REG glycerol (REG-80) for 24 h.
Despite the huge amount of exposed proteins and pathogens analysed, around 2000, we could only consider approximately 20 protein alignments, representing only 1% of the total of proteins analysed.
Table 1 shows the total of proteins of groups (i) and (ii) based both on the predicted and the experimental proteomes.
Using the sum total of proteins predicted by the other ant genome projects, and 886,838,537 mapped reads, automatic annotation was then carried out using the MAKER2 pipeline.
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No measurements of the total amount of proteins in the cells or the overall rate of the protein synthesis were made in this experiment.
The summation of the number of replicated proteins was defined as the total amount of proteins.
All the membrane mixtures contained the same total amount of protein.
Cyclic voltammetry indicated surface concentration of electrochemically active cytP450 around 0.6 nmol/cm2, which corresponded to 5% of the total amount of protein that was consumed by the immobilisation process.
Also, the total amount of protein as a function of cell number was determined.
Results were expressed by normalizing peak areas by the total amount of protein obtained.
The total amount of protein in the supernatant was measured according to the Bradford method.
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