Library campaigners accuse the government of "hiding" the scale of cuts which they predict will force the closure of a further 400 UK libraries by 2016, bringing the total of library closures since 2009 to more than 1,000.
All three cell lines were run as SureSelect and SeqCapEZ libraries, for a total of six libraries that were sequenced in a single lane of a 100 bp paired-end run on a HiSeq 2000.
The total number of libraries and mobile lending points dropped by 71, down 1.7%, to 4,194 on a yearly basis.
The annual survey by the Chartered Institute of Public Finance and Accountancy, released officially tomorrow, shows a continued fall in library branches in the year to March 2014, with the total number of libraries in the UK now standing at 4,145.
This limited the total number of libraries to six glioblastoma and nine astrocytoma libraries containing 487,197 and 863,610 SAGE tags each, respectively.
Tissue samples per block (4 subsamples) were pooled for a total of 8 libraries prepared for each tissue (16 libraries total).
A total of 4 libraries were produced: branchial plume vs. body wall subtracted library (and its reciprocal) and trophosome vs. body wall subtracted library (and its reciprocal).
We prepared a total of ten libraries from these DNA samples, by pooling 3 strains each in eight libraries, and 4 strains each in the remaining two libraries.
Three libraries (large, medium, small) were constructed for each feeding phase resulting in total of 12 libraries.
The Ion Xpress™ Plus Library Kits and AB Library Builder™ (Ion Xpress Plus Library Protocol v. 1.00) were used to prepare one library for each animal's pool of mutants from purified PCR products (for a total of 24 libraries).
They show that a net total of 106 libraries closed in Britain in 2014-15, a fall of 2.6%, from 4,023 to 3,917.
