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Human samples; 100 µg of total protein total obtained from protein lysates was separated on 12% PAA-gels, transferred onto PVDF membranes and stained using rabbit anti-mouse monomeric TRAP or rabbit anti-rat total TRAP as described above.

A database survey of 16S rRNA gene sequences (585 records in total) obtained from olive mill waste environments revealed the dominance of members of Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria, Firmicutes, and Actinobacteria.

Each human protein was used as query in a homology search against a database containing the proteomes of 1,074 eubacteria and 82 archaebacteria (3,792,506 sequences in total), obtained from the National Center for Biotechnology Information in August 2010 (supplementary table S1, Supplementary Material online).

(B ) Percentage of deep sequencing reads matching vsiRNAs in total reads obtained from total extracts, AGO1a, AGO1b, and AGO18-associated small RNAs.

(A ) Percentage of deep sequencing reads matching the indicated miRNAs in total reads obtained from total extracts, AGO1a, AGO1b, and AGO18-associated small RNAs.

The total pore volume (V total) was obtained from the amount of nitrogen adsorbed at P/P 0 = 0.99.

The gel of total proteins obtained from sugar cane bagasse showed a similar standard of bands compared to total protein gel obtained from the cellulose enzyme broth.

Cognitive outcome was measured as the total scores obtained from comprehension post-tests.

Total scores obtained from factor analysis and measured scale values are in good agreement.

The assessment of the best probability distribution was based on the total rank obtained from all the tests.

This result corresponds to 0.15% and 0.09% of the total sequences obtained from the BA and RJ samples, respectively.