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We normalized coverage by dividing coverage depth by the total number of reads in the metagenome.
These counts were then added to generate a total number of reads mapping to each gene.
Average and total number of reads for each experiment and single condition are listed in Supplementary Table 2.
Simultaneously, the total number of reads used and the total number of correctly classified reads were tracked.
The expression levels were normalized by dividing the matching reads by the total number of reads obtained for each sample, multiplied by two times the average read length.
Total number of reads and coverage analysis of Mendeliome data.
Total number of reads that can be aligned to this locus.
australiensis Total number of reads: 135,769; Total reads are repetitive: 86.35%; Reads that are 70 100% repetitive: 73.06%.
The total number of reads used in the final assembly was 25,531.
Remarkably, these planktonic OTUs are not very numerous (1.4%), yet they contribute almost 40% of total number of reads.
We then scale the values such that the correction is neutral with respect to the total number of reads.
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