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We also compared the total number of assembled bases for each assembly.
Thus, the performance of transcriptome assembly methods was evaluated by the total number of assembled transcripts matching UCSC annotations.
To assess Cufflinks and genome-guided Trinity assembly performance, we used Cuffcompare to calculate the total number of assembled transcripts, the number of intergenic transcripts, and the number of antisense transcripts [ 74].
A range of k-mer values were tested, and a final assembly was accepted on the basis of features such as total number of assembled contigs, N50 value and cumulative contig length.
b Total number of assembled clusters.
#Total number of assembled read bases/Total number of bases in consensus sequence.
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After hierarchical clustering total numbers of assembled transcripts were reduced from 62,065 to 29,603 wherein 14,855 transcripts had lengths above 1000 bp (Additional file 3).
Thus, the total number of sequences assembled into isotigs was 84,289.
a Total number of ESTs assembled b Unigenes (singletons and contigs) from a single library a Number of motif repeats found in the Citrus protein set.
The resulting sum was divided by the total number of reads assembled to obtain the percentage of reads matching their original genome.
The total number of sequences assembled to form each contig varied for all five PALs reflecting variation in their respective expression patterns [ data not shown].
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