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The EGCG restored arsenic induced decrements in epididymal sperm concentration, kinematic attributes (total motility, rapid, progressive motile, fast progressive, VSL, VAP, VCL, BCF, LIN, WOB, STR and Type A), structutal membrane integrity, functional membrane integrity and mitochondrial membrane potential.
The post-thaw motility was evaluated with a light microscope for the percentage of progressively motile spermatozoa, total motility percentage and a velocity score (from 1 to 3).
Computer-assisted sperm analysis measured progressive and total motility, velocity, and linearity.
The percentages of total motility and progression were equally enhanced in heated sperm for the two wash methods.
However, compared with placebo, nutraceutical supplementation increased (P <.05) total motility, trajectory speed, as well as plasma and acrosomal membrane integrity in spermatozoa from fresh semen.
In cooled semen, nutraceutical treatment also increased (P <.05) total motility, speed, and membrane integrity of spermatozoa compared with the control.
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While semen volume was similar between fertile and infertile men, sperm density, motility, and total motile sperm count was significantly lower for infertile men (Table 2).
ANOVA and linear regression were used to determine correlations between AGD, fatherhood status, and semen analysis parameters (sperm density, motility, and total motile sperm count).
We observed significant positive associations between AGDAS and sperm concentration, motility, morphology, total sperm count, and total motile count.
Results: AGDAS, but not AGDAP, was associated with sperm concentration, motility, morphology, total sperm count, and total motile count (p-values, 0.002 0.048).
AGDAS was positively related to sperm concentration, motility, morphology, total sperm count, and total motile count (p-values 0.002, 0.028, 0.048, 0.006, and 0.009, respectively; Table 3).
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