Sentence examples for total messenger from inspiring English sources
Four micrograms of total messenger RNA (mRNA) were reverse transcribed using superscript II reverse transcriptase and oligo dT primer (Invitrogen).
Total messenger ribonucleic acid (mRNA) was isolated from MDA-MB-231 tumour cells or tumour tissue using Trizol Reagent (Invitrogen, Carlsbad, CA, USA) according to the manufacturer's instructions.
The total messenger RNA (mRNA) from cellular samples was extracted using the TRIzol lysis reagent (Invitrogen, USA) according to the manufacturer's protocol.
Total messenger RNA was isolated from the epidermis only, using GenElute Mammalian Total RNA Miniprep kit (Sigma Aldrich).
Total messenger RNA was isolated using Trizol reagent (Invitrogen) according to the manufacturer's instructions.
Total messenger RNA was extracted and converted to cDNA pools using reverse transcriptase PCR.
Most DNA based microarrays are used for the analysis of the total of messenger RNAs (transcriptome) and provide a snap-shot of what's going on in a cell population at the time of sampling.
Total and messenger RNA quantities and quality were assessed by Nanodrop and Bioanalyser using RNA chips for Agilent 2100 Bioanalyser (Additional file 1).
The yield of RNA measured by a quantitative PCR assay for GAPDH was higher with total than messenger RNA (median 27-fold, interquartile range 7.6- to 101-fold), suggesting that RNA degradation may indeed be a problem in the biopsies.
The transcriptional levels of these two genes were estimated to be 6 and 32000 copy number, respectively, so FD transcripts represented nearly 0.02% of the total grapevine messengers.
Calcium-dependent glutamate secretion was reconstituted in Xenopus oocytes by injecting the oocyte with total rat cerebellar messenger RNA (mRNA).
