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The primary, secondary and total medium effects are calculated.
After 24 h, medium was replaced with WEM supplemented with Cell Maintenance Supplement Pack (Invitrogen) and total medium was replaced every 48 h.
In addition, the simulation results showed that sedimentation of cells more likely occurred at the bottom of the ORP with a total medium depth of 0.2 m than at 0.3 m at the same inlet velocity.
Simulations were further conducted on a large-scale ORP with 100 m2 surface area operated at total medium depths of 0.2 and 0.3 m, average cell concentration of 0.4 g/L, and inlet velocities of 0.1, 0.2 and 0.3 m/s from the paddlewheel.
In the example, this is shown on the row labelled "Total medium effect with 80% usage" for the non-usage, and on the row "Total medium effect with 60% penetration rate" for the penetration rate.
Secondly, the total effect was computed by multiplying the coefficients for each mechanism and giving this total effect as percentage, on the row labelled "Total medium effect".
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Table 1 Adapted from the European commission [3] Company category Employees Turnover or balance sheet total Medium-sized <250 ≤€50 m ≤€43 m Small <50 ≤€10 m ≤€10 m Micro <10 ≤€2 m ≤€2 m.
A cDNA library was constructed from the total conditioned medium RNA using a random priming technique in a pcDNAII vector.
He did not think of photography as a "total" artistic medium, the way drawing and painting were, but his photographs are his best work, maybe because it's the work he cared less about.
"Our goal is a total print medium where your paper is going to talk," says Andrew Ferber, vice chairman at T-Ink and one of the brains behind the technology.
It is of interest to note that in some cases the introduced deterioration was negative, which is reasonable since in the calculation of total deterioration, medium and large tears and missing pieces were added up.
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CEO of Professional Science Editing for Scientists @ prosciediting.com