Thus, the total magnification was 32×.
Samples were analyzed at a total magnification of 400 ×.
An unbiased counting frame was used, and counting was performed using an × 20 lens at a total magnification of × 794.
Deposited fibres, recognized as being the main factor leading to high surface coverage [16], were counted manually under a binocular magnifier Leica MZ10 (total magnification: 110 ×). 3.
b Microscopic picture of the purulent material showing long, slender acid fast bacilli along with polymorphonuclear cells (Ziehl Neelsen stain, total magnification ×1,000).
1. Surface coverage was evaluated with Histolab Microvision® on 10 random observation fields (Leica Leitz Laborlux 12POLS microscope linked to a camera, with a total magnification of 526, thus a 2.4280 mm2 surface per glass).
The total magnification of XDM (~440×) is the product of FZP magnification (~22) and optical magnification, producing an effective pixel size of 15 nm on the sample and a field of view of ~15 × 15 μm2.
Surface coverage was evaluated with Histolab Microvision® on 10 random observation fields (Leica Leitz Laborlux 12POLS microscope linked to a camera, with a total magnification of 526, thus a 2.4280 mm2 surface per glass).
Immunostained tissue sections were first assessed at 100× or 200× total magnification and 0.8 numerical aperture of the objective lenses.
The cells were viewed by fluorescence microscopy at 630× total magnification on a DM 4000B fluorescence microscope (Leica, Germany).
Insert membranes were isolated following migration and stained with DAPI to allow for quantification of migrated cells (total magnification, 100×).
