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Also, the proportion of the B-lymphocytes among the total lymphocyte population was increased, suggesting that a humoral response developed, and no change was observed in the γω subset.
CD4 expressing T-cells were expressed as percentage of the total lymphocyte population and age adjusted cut-offs were used to define immunodeficiency[ 40].
We demonstrate that malignant cells, comprising as little as 1% of the total lymphocyte population are detectable, using SOX11 as the sole discriminant antigen.
CD4-positive lymphocytes are determined by gating the CD4-bright population using a CD4-PE (logarithmic scale) against side-scatter (linear scale) dot plot of the total lymphocyte population (Fig. 1d).
Samples from five patients with CLL were serially diluted in normal leukocytes, such that the CLL cells represented an actual tumor burden as low as 0.01% of the total lymphocyte population.
CD4-positive lymphocytes are then determined by gating the CD4-bright population using a CD4-PE (logarithmic scale) against side-scatter (logarithmic scale) dot plot of the total lymphocyte population (Fig. 1b).
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Following on from our recent validation of finger-prick blood samples for CD4-lymphocyte determination in adults (11), we encountered no difficulties in labeling and gating CD4-lymphocyte and total lymphocyte populations for the determination of %CD4/lymphocyte values in finger-prick samples taken from children using the Blantyre count method with the EPICS XL-MCL flow cytometer (Figs. 1e and 1f).
Results are presented as the percentage of cells expressing intracellular cytokines in total lymphocytes population.
Results were expressed as numbers of cells per microliter of blood for lymphocyte subsets and as percentage of positive cells among total CD4+ lymphocyte population for Treg.
Consistent with their phenotype, sooty mangabey NKT lymphocytes were significantly enriched for CD8+ T lymphocytes and had significantly lower number of CD4+ T lymphocytes compared to the circulating total T lymphocyte population (Table 1).
However, IL-6 protein expression by lymphocytes isolated from low-fat-fed C57BL/6 mice was elevated following 96 h of activation by CD3/CD28 stimulation, which results in activation of the total T lymphocyte population.
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