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We compared the i) percent of reads remapped to the transcriptome (completeness); ii) the percent of correctly oriented mapped read pairs (accuracy); iii) total length of assembly and mean length of assembled transcripts (contiguity); iv) number and length of predicted ORFs, and v) number of orthologs identified using reciprocal Blastp against other validated protein sequence databases (Table 1).
The number of contigs after clustering, total length of assembly and average length of transcripts is shown.
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The total length of assembled bases and the median contig length of the assembly have also been indicated in Table S6.
The N50 length now reached 190,514 bp, and 77% of the total length of the assembly was assembled into regions larger than 50 kb (as compared to 3% before running the software).
Although most of the parameters (e.g. N50, Maximum length, Average length, Contig number and Total length) of the assembly result using raw data were the largest among the three assembly results (Table 1), the results may contain inaccurate sequences due to the existence of poor quality bases in the raw data (Additional file 3).
The total length of the assembly contained in gaps decreased from 15.93 to 6.47 % of the assembly length, a 59%% improvement.
Sequence assemblies obtained by DBG and OLC approaches were quality assessed using standard assembly metrics such as N50, total number of contigs, and total length of the assembly (supplementary file S2, table S1, Supplementary Material online).
Genome sequences were quality controlled before the pan-genome construction by making sure that the total length of each assembly was between 2.0 and 2.3 Mb (the common genome length of completed S. pneumoniae genomes as retrieved from https://www.ncbi.nlm.nih.gov/genome/).nih.gov/genome/
The top half of the contigs (1,630 clones) contained 69% of the total length of the assembly.
The total length of the assembly was 1,370,818 nucleotides, consistent with the sizes of other A. phagocytophilum genomes.
Fifty percent of the total length of the assembly was contained in the contigs or scaffolds with lengths of ≥30 kb (i.e. N50 = 30 kb).
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