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The amounts of the products (i.e. bands smaller than the end-labeled primer) were determined relative to total labeled products seen using a phosphorimager, after subtracting the background from the – PPi lane.
Tunel-labeled cells were counted and averaged as total labeled cells per high magnification field, according to experimental groups.
Repressing FoxC2 activity with a dsRNA or with a dominant negative FOXC2-Engrailed fusion protein resulted in an increased proportion of GFP-labeled, desmin + myotomal cells out of total labeled cells, at the expense of SM.
Labeled claudin-4 was present in the media as early as 2 hours post-labeling and reached a level of approximately 3 4% of the total labeled claudin-4 after 24 hours.
The amount of degraded support (Ds) corresponded to mean difference between original and final dry weight (g) of labeled SS without fungus was determined from D{text{s}} = frac{1}{N}mathop sum limits_{n = 1}^{N} left( {W_{0} - W_{text{f}} } right), n,where W 0 and W f are the original and final dry weights of each labeled SS (n), respectively, and N is the total labeled SS used in the test.
Amounts of the excised products (<15-mer) were determined relative to total labeled products.
Similar(36)
Good candidate viruses would be RNA viruses (not retroviruses) since it would be easy to prepare total host DNA, treat it with RNase and probe it by dot blot for positive hybridization with the total, labelled viral cDNA.
The mean percent ± SD of co-labeled puncta were expressed relative to the total Glut3 labeled puncta and to the total rab11 labeled puncta, respectively.
At harvest in 2011, in mulched treatments the total labeled-N remaining in the 0−170 cm soil layer was 25% greater whereas unaccounted labeled-N was 69% less, than in non-mulched treatments, regardless of whether maize was cropped.
At harvest in 2012, plastic mulch increased total labeled-N remaining in the 0−170 cm depth in cropped soils and unaccounted labeled-N in non-cropped soils, compared with no mulch.
AVA consists of more than 57,000 video segments labeled with 96,000 labeled humans and 210,000 total labels.
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