The proportion of denitrifying bacteria in the total isolates was more than 6-fold larger with FSC isolation (57.1%) compared with the APD method (9.2%).
As a general rule the MICI/MICPAβN quotient ranged from 4 to 16 (267 isolates, 77.8% of total isolates).
Fusarium (Nectriaceae), Alternaria (Pleosporaceae), and Cladosporium (Cladosporiaceae) were the most abundant genera, accounting together with 43% of total isolates (Supplementary Fig. S2; Table S1).
trehalosi 2) constituted 36% of the isolates, and were the only biovariants sufficiently numerous to account for >7% of the total isolates.
Multidrug resistance was also observed in 55.2 % of the total isolates.
Ten of the strains showed inhibition to HepG2 cancer cell line in varied degrees, accounting for 71%% of total isolates, 3 strains exhibited antibacterial activity and one showed acute cytotoxicity and wide-spectrum of antibacterial activities.
In a study performed in 2006 in 11 Portuguese hospitals scattered all over the country, EMRSA-15 accounted for 54% of the total isolates [25].
For instance, in South America isolates from the A.Br.003/004 sub-group (mean within-group VNTR distance = 0.08; Fig. 3A) comprise more than 80% of the total isolates from this region yet are rarely observed elsewhere in the world.
In total, 122 isolates from the environment and one N3 isolate (11.1% of subtype and 0.82 of total isolates; Table 2 and Table S1) had R152K (zanamivir-related) (Table 4).
Remarkably, this analysis disclosed that 348 (96.4%) of the 361 isolates belong to the Euro-American super-lineage [27], which was itself strongly predominated by the LAM lineage represented by 241 (66.2% of the total) isolates.
The total isolates available for serogrouping was 428.
