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Z-plans (2 μm/section, 31 sections in total) were acquired every 10 min for 4 h.
At each of the six sample points, 10 navigators (of 30 s duration in total) were acquired to check for noise in the navigator-to-navigator registration.
For retinal imaging, six raster scans with orthogonal fast scan axis orientation (horizontal and vertical) each consisting of 700 × 700 axial scans requiring an acquisition time of ~5 seconds for each raster (~30 seconds total) were acquired.
Repeat this procedure until total integration time is acquired.
A total of 13 total slices were acquired, and a max projection image was created for analysis of foci using ImageJ (NIH) to calculate the distance between SPBs in large budded cells.
However, the deal mirrors another acquisition of a former YC company last year, Heyzap, which had raised $8 million in total, and was acquired by Germany company RNTS Media, for up to $45 million.
2×105 to 3×105 total events were acquired on a FACSCanto II flow cytometer and analyzed with FACSDiva 6.1.2 software (both BD).
Between 8 and 10 million total counts were acquired.
The total score was acquired by adding respective points which were then expressed as mean percentage.
The total luminescence (total cell number) was acquired through adding 50 µl of lysis reagent/well to lyse all cells.
In total, 26 datasets were acquired over the two day test period.
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