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Overall, there was only a small increase in the proportion of total interspersed repeats between the reduced set (2.34%) and the unannotated set (2.90%).
The total interspersed repeats represented 36% of the intergenic transcripts (Additional file 3: Table S4), a percentage higher than those observed in previous porcine transcriptome analyses: 5.8%-7.3% [ 22] and 7.3% [ 28].
In total, 15.3 Gbp (65.34%) of genomic sequence was labeled as interspersed content via homology (Table 4), while de novo estimates place total interspersed content at 79.3% (similarity and de novo combined in bin 1 yield 81.81%).
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Each run was 9.6 min long and consisted of 16 blocks of stimuli (4 s/stimulus plus 0.5 s prestimulus fixation, total 18 s) interspersed with 16 blocks of rest (18 s).
The ratio of the total length of known interspersed repeats to the total genome length ranged from 0.35% (FANhybrid_r1.2) to 0.94% (FAN_r1.1).
We next used the same reagents and equipment to perform qPCR using each primer pair on three technical replicates of the three biological replicates from both strains (18 samples total), with the samples interspersed on a 96-well plate to avoid batch effects.
The mitosis:meiosis cycle was repeated 12 times, for a total of approximately 300 mitotic generations interspersed with 11 rounds of sporulation, which included outcrossed sex for the appropriate treatments.
Using various bioinformatics tools to identify repeats de novo, we found a total of 8,162 putative interspersed repeats divided into 456 consensus interspersed repeats for PILER, 5,440 repeats for RepeatScout, 1,977 LTR elements from LTR_finder and 289 LTR elements using LTR_STRUC (Additional file 1).
Sums of the numbers in the columns do not match the totals because some sequences had two different interspersed repeats within the 100 bp.
In the MRI experiment, participants performed five blocks using each auditory stimulus tapping rate combination for a total of 20 sensory-motor tasks that were interspersed (see below) with no-movement runs; the no-movement runs were verbally instructed by the command "no movement" (see Fig. 1B).
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