Sentence examples for total glut from inspiring English sources

To measure the total GLUT content, cell lysates were subjected to 10% SDS-PAGE and Western blotting (19).

Furthermore, treatment with GL extracts promoted IRS-1, AKT, PI3Kp85 expression, then IRS-1, AMKP, and AKT308, but not AKT473, phosphorylation, accompanied by increasing the ratios of membrane to total Glut 4 expression and adiponectin receptor 1 transcription in the skeletal muscles.

Twelve weeks following ovariectomy, total GLUT-4 protein levels in soleus muscle were reduced by 33% in OVX rats.

Thus, the effects of C. comosa and compound 049 treatment on insulin-stimulated glucose transport activity and on total GLUT-4 protein levels in skeletal muscle were studied.

To determine the contribution of the major protein degradation pathways to the decrease in the total GLUT-1 protein content found in normal chondrocytes exposed to high glucose, specific inhibitors of the proteasome (MG-132) and lysosome (chloroquine) were used.

In contrast, normal chondrocytes responded to high glucose by decreasing the 2-DG uptake and the total GLUT-1 content, suggesting that downregulation of GLUT-1 mediates the decrease in glucose transport.

Glucose deprivation for 48 hours significantly increased the total GLUT-1 protein levels both in normal chondrocytes and in OA chondrocytes, relative to their respective controls cultured under RGM for the same period.

To ascertain whether the differences in total GLUT-1 protein content induced by culture of normal and OA chondrocytes under high glucose were due to alterations in GLUT-1 gene expression, quantitative real-time RT-PCR analysis was performed.

The total GLUT-1 protein content was markedly decreased in normal chondrocytes incubated with 30 mM glucose for 18 or 48 hours, but remained unchanged in OA cells cultured under the same conditions (30 mM glucose), relative to those cultured in RGM, independently of the duration of exposure to high glucose.

Total cellular GLUT 4 protein content was determined using a monoclonal anti-COOH-terminal GLUT4 antibody (kind gift, G.D. Holman, Bath), as described previously [ 34].

**p < 0.01, ***p < 0.001 B. Vemurafenib induces increase in total membrane GLUT-1 expression (green) in immunofluorescently stained sections (blue = hoechst nuclear stain), shown at 4× magnification.