Tumors were homogenized and processed to obtain total fractions for western blot as described previously [41].
Fractions 9 and 10 were combined, yielding 11 total fractions for subsequent LC-MS/MS analysis.
As the spectrum of this type of proteins only changes when they assemble into filaments, one can use the magnitude of this spectral shift as a measure for the total fraction of proteins that have self-assembled.
Our results also hold for reference models quantitatively different from the original Connor-Stevens model both for the total fraction of temperature-compensated models RMSD <0.5) and the strong influence of potassium channel dynamics on the temperature dependence of firing rate, 'Materials and methods' for details.
The limits of quantification for T4, rT3 and T3 amounted respectively 0.04 ng mL−1, 0.05 ng mL−1, 0.03 ng mL−1 for the total fraction, and 6.6 pg mL−1, 2.6 pg mL−1 and 2.7 pg mL−1 for the free fraction.
Supernatant was collected as total fraction and centrifuged at 12 000× g for 15 min at 4 °C.
In this respect, multiple-steps growth kinetic (performed at low MOI) showed the viral titres in both extracellular and total fractions were larger for N569 than for A663, supporting the contribution of cell-to-cell spread to the lysis plaque area reduction observed for the A663 strain (data not shown).
A maximum of 30% of total added CBH I was detected in the supernatant, which is nearly half of the total recovered fraction for AFEX-CS.
The linear regression on all the simulated infarctions was y = 0.97x – 0.01; R2 = 0.94. Figure 8 Correlation between AIF and the total infarcted fraction for various infarction geometries.
We found that the average total contact fraction for cemented components was 9.5% and had a power law response (decrease) with years in service.
This experimental curve was very close to the theoretical predictions derived from the numerical ventricle model (Figures 6 and 7). Figure 8 shows the correlation between AIF and the total infarcted fraction for different surrounding background levels and for different defect polar positions versus the polar map segmentation and versus the breathing directions.
