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Bioavailable, exchangeable and total forms of Cd, Co, Cu, Zn, Pb, and Mn were determined.
Each slide was then probed with one of 60 different antibodies specific against phosphorylated or total forms of signaling proteins.
Antibodies against phosphorylated (active) and total forms of different members of Smad-dependent and Smad-independent signaling pathways were used.
The phosphorylated and total forms of JNK MAPK were detected with rabbit anti-mouse polyclonal antibodies (Cell Signaling).
Primary antibodies against phosphorylated (P-ERK1/2) and total forms of ERK1/2 were purchased from Cell Signaling Technology (#9101 and #9102 respectively).
In order to validate the RPMA data, the levels of eight selected proteins were analyzed by Western blots using antibodies against phosphorylated and total forms of proteins (Fig. 4).
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This derivation was achieved by expanding the total form of the constitutive equation by a first-order Taylor series, with respect to the stress, creep, and shrinkage strains, and the elastic modulus of concrete.
The same membrane was processed in three sequential steps: incubation with (i) antibody against phosphorylated form of ERK1/2; (ii) antibody against the total form of ERK1/2; and (iii) antibody against α-tubulin.
All p-MET positive cases expressed the total form of the receptor.
However, no influence on the total form of β-catenin was detectable.
SEM, RS4 11 and Jurkat cells showed a decrease of the phosphorylated as well as the total form of 4EBP-1 anter an incubation of 4 and 24 h.
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CEO of Professional Science Editing for Scientists @ prosciediting.com