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Pearson correlation coefficients and linear regression were used to describe the association between FN based on field area(s) and total FN in the muscle cross-section.
We examined the association between FN in 1, 2, 3, and 4 fields and the total FN in 23 rat muscle cross-sections in order to determine whether all 4 fields were needed to better predict total FN in a cross-section.
FN from any 3 of the 4 fields was a better predictor of total FN than that from 1 or 2 fields (Table 1), with correlation coefficients ranging from 0.57 to 0.59.
FNs from 4 fields did not further improve the correlation coefficient with total FN (r=0.57).
This goes along with a sHA1-induced significant shift towards DOC-soluble FN (about 80% of total FN was DOC-soluble) in comparison to CTRL (about 40% of total FN was DOC-soluble) (Fig. 5C).
The total FN nanofiber volume was measured as the summation of the volume of each pixel within the masked area.
Similar(36)
Similar to the staining pattern previously obtained on these same samples with anti-total-FN antibody [ 15], anti-N-terminal-HBD mAb was observed to produce preferential staining of the ~200+- as compared with the ~170-kDa species in both OA and RA SF samples.
Despite the use of gradient gels with the capacity to resolve species as small as ~15 kDa, little or no staining of forms of FN smaller than ~170 kDa was detected in unconcentrated SF samples by anti-N-terminal-HBD mAb (or anti-total-FN polyclonal antibody; not shown) after autoradiogram exposure times of 5 days (Fig. 2).
BMD: Bone mineral density BMC: Bone mineral content LS: Lumbar spine TH: Total hip FN: Femoral neck TR: Trochanter OC: Osteocalcin ucOC: Undercarboxylated osteocalcin TRACP-5b: Tartrate resistant acid phosphatase isoenzyme 5b Values of bone densitometric measures represent mean ± SD.
mRNA expression of total and cellular FN (EDA-FN or EDB-FN) were also not altered (Supplementary Figure S4).
As seen in Fig. 5B, the increase of total amount of FN (sum of DOC- and SDS-fraction) is mainly the result of increased DOC-soluble, less assembled FN.
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