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The frequency of yielding inheritable HDR modifications ranged from 0.8%to1.4%4% in F1 flies, and the frequency of homologous recombination (HR) yielding F0 in total fertile F0 ranged from 4.6%to5.8%8% in F0 flies.
The efficiency of obtaining the yellow white-KI flies was 0.5% in germline transmission, and the frequency of HR yielding F0 in total fertile F0 was 1.3% as shown in Table 1.
We did this and analysed data for a period representing the total fertile phase of the cycle [the ovulation window plus the two preceding days to allow for sperm longevity, see e.g. [ 14]] and a 10 day period surrounding the fertile phase (5 days before, 5 days after).
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Total numbers of fertile eggs were correlated with total lifespan using Spearman's rank correlation coefficient.
A total of 6846 fertile men were invited and 792 participated.
A total of 33 fertile mutants with stable visible phenotypes were crossed with wild-type males and classified.
We isolated five carriers from 1 founder out of a total of 56 fertile G0 flies, resulting in an HDR efficiency of 1.8%.
A total of fourteen fertile transgenic plants were obtained carrying bp100.2i (2 independent events), bp100.1 (9 independent events) or bp100.2mi (3 independent events).
A total of 72 fertile crosses involving virgins (in pairs) or single males mated with y w; al b c sp/SM6a flies could be set up.
The total number of fertile eggs produced during the entire lifespan was 291 for strain N2 and 289 for strain MY6.
A total of 1500 fertile chicken eggs were divided randomly into five treatments (300 eggs each): control was maintained at 37.8 °C and 56%% relative humidity (RH) whereas, TM1, TM2, TM3 and TM4 were subjected to 38.5, 39, 39.5 and 40 °C for 18 h and 65 % RH daily during embryonic days ED 12 18.
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