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Results are expressed as percentage of total enzyme content of Triton X100-treated cells.
β-hexosaminidase release into the supernatant was measured by enzymatic cleavage of the fluorogenic substrate 4-methylumbelliferyl-N-acetyl-β-D-glucosaminide and expressed as % of total enzyme content of Triton X-100-treated cells [19].
β-hexosaminidase release is expressed as a percentage of the total enzyme content of Triton X-100-treated RBL-2H3 cells.
In brief, RBL-2H3 was passively sensitized with diluted murine serum IgE, and mediator release was triggered by the addition of antigen at 0.3 μg/mL and is expressed as percentage of total enzyme content of Triton X100-treated cells [ 12].
This is a result of the high expression levels of mucin glycan degrading families 2 and 20 β-N-acetylhexosaminidases (EC 3.2.1.52) in FS-11 with respect to the level of total GH-like enzymes (Fig. 4B) and total enzyme content (Fig. 4C).
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However, CCR may not be the only mechanism that may contribute to the maintenance of intracellular MC by reducing the need for increased total protein (enzyme) content of the cell at rapid growth.
Hydrolysis simulations were performed based on the experimental conditions: weight of solution (scale of hydrolysis), solid loading, cellulose content, total enzyme loading (mg protein/g cellulose), ratio of enzymes present (EG CBH I CBH II:BG), temperature, pH and hydrolysis duration.
Specific activity was defined as a rate of total enzyme activity over protein content in milligram.
Hydrolysis simulation was initialized based on the weight of solution (scale of hydrolysis), solid loading, cellulose content, total enzyme loading (mg protein/g cellulose), ratio of enzymes present (out of eight classes), temperature, pH and simulation time (hydrolysis duration).
There was no relation between total carbohydrate/sugar and enzyme contents, except of wild grasses and wheat bran.
Fed-batch SSF was initially started as a batch SSF of 500 g as a working weight with 10% (w/w) WIS content, 50% of total yeast cell suspension (fed-batch adapted) and 50% of total enzyme preparation.
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