Single transfection and co-transfections were carried out using 3 μg of plasmid(s) of interest in total per dish, supplemented with 8 μl of GeneJuice.
Taking it to the extreme is Le Relais de Venise in Manchester or London, which has a grand total of one dish on the menu.
Ten colonies from each dish (total 20 colonies) showing lower GFP expression were picked up for further expansion in ES medium.
They were plated in a final volume of 1 ml at a concentration of 5×104 total BM cells/culture dish (Falcon 1008) for murine and 5000 CD34+ cells/ml for human progenitors.
Data were expressed as relative LDH activity, calculated by dividing LDH activity by the total protein per dish.
The cell suspension from each CNS was distributed equally into six culture dishes and the total volume in each dish was brought to 100 μl with culture medium.
Three dishes per gap × ten gaps gave a total of 30 dishes and 300 seeds per species.
We asked for a total of six dishes, leaving it up to the chef to coordinate our tasting menu.
It wasn't what you'd call a recipe, but it brought the total of savory dishes to five.
The set menu works best for groups of two, which allows the tiny kitchen to turn out a total of eight dishes.
A team of top chefs are hoping to set a new world record by preparing a total of 105 dishes in one day from one single giant cabbage.
