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After a total cultivation time of 48 h the 1023.βA reached a maximum value of β-amyrin of 0.69 mg/L (Figure 8).
The culture broth was transferred into fresh minimal medium, and the test-tube cultivation was repeated 22 times for a total cultivation time of 445 h.
However, when glucose was utilized, the total cultivation time and l-lysine production remained the same, even though intracellular H2O2 concentrations decreased (Table 5).
After an additional dsRNA feeding pulse within a total cultivation time of 57 hours, the relative actin mRNA levels remained within a reduced range of 76.9% in relation to control animals.
After 24 hours total cultivation time, the serum-free medium was renewed and chemically synthesized siRNA for Tgif1 (20 nmol) was transfected with INTERFERinTM purchased from Peqlab (Erlangen, Germany) according to the manufacturer's instructions.
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The fraction of plasmid-harbouring cells in the total population z versus cultivation time is shown in Figure 2 for all investigated plasmids.
As evidence for our hypothesis, P. purpurogenum IAM15392 was transferred to fresh basal medium after it was cultured in medium containing glutamine and then was grown until the cultivation time reached a total of 96 h.
∅ DWrepresents the mean biomass concentration during the given time interval, V represents the total working volume, and t represents the cultivation time.
The chronosequence of soil development was obvious in terms of increasing the contents of SOC, nitrogen, phosphorus, DTPA-Mn, DTPA-Zn, and DTPA-Cu with cultivation time, and decreasing soil pH, salinity, and the contents of potassium, total Mn, total Zn and total Cu with cultivation time except for no obvious decreases of total Cu in the cotton barley cropping systems.
The complete set of DNA array data was available in Additional file 8. Microarray analysis showed that a total of 2215 genes were differentially regulated at transcriptional level in at least 1 cultivation time point.
Total RNA was isolated from the mycelial samples of three parallel cultivations collected at the cultivation time points 3 and 5 days using Trizol™ Reagent (Gibco BRL), essentially according to manufacturer's instructions.
Related(20)
total harvest time
total incubation time
total culture time
total interview time
total production time
total training time
total flight time
total sintering time
total sampling time
total intervention time
total treatment time
total acquisition time
total analysis time
total exercise time
total cultivation volume
total kneading time
total cultivation period
total integration time
total setup time
total preparation time
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