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Control males directed wing extensions to another male 0.7% (±0.5) of the total courtship time, whereas males with activated OA neurons courted the second male 25% (±4) of the total courtship time (Fig. 1A).
Examining multiple parameters of male courtship behavior indicated that total courtship activity was not significantly altered in OA-neuron-activated flies.
We then conducted a one-sample t test versus the null expectation that one-sixth (60 out of 360°) of total courtship time should be spent upstream of the female.
We continuously monitored the videotape for male-female courtship interactions [40] and scored both total courtship time and the amount of time that males were positioned upstream of females, operationally defined as whether the male was oriented within approximately 60° of the prevailing current with respect to the female.
We also recorded whether females were moving or stationary; they were immobile for one-third of the total courtship time.
We quantified 30 ethograms of completed courtships (Experimental Procedures) in wild-type Oregon-R flies for courtship behaviors including wing fluttering alone (about one-third of total courtship time), abdominal quivering alone (one-seventh of total courtship time), and simultaneous wing fluttering and abdominal quivering (one-seventh of total courtship time).
Similar(51)
The Cpi calculates the total relative courtship directed at each target and is defined as Cpi = (CIf − 0.5)/ CIf + CIm), where CIf and CIm represent the fraction of time courting the female and decoy male, respectively (Villella et al. 1997).
The total time showing courtship behavior in the preference zones varied considerably among individuals.
Specifically, a one-way ANOVA was used to partition the total variance of courtship intensity into the variance among males (SA) and the variance within males (S).
During each observation, a vial was observed for 30 seconds and total number of courtship events (chase, wing-flap, mounting-attempt etc).
'Male ID' explained 69.0% of the total variance in male courtship, while 'female ID' (as a source of within-male variation under special interest) explained 6.0% and 'day' explained 1.6%.
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