∑Q−, where ssf represents the section sampling fraction, asf is the area sampling fraction, hsf is the height sampling fraction, and ∑Q− is the total count of nuclei sampled for the entire layer (West, 1999; Dorph-Petersen et al., 2001; Schmitz and Hof, 2005).
The total count of LAB in these samples was approximately 7.00 log cfu/g after ripening process and 6.00 log cfu/g after storage time.
Percentage of phosphorylation was calculated by dividing the bound radioactivity by the total count of (γ-32P ATP per sample.
Read counts were then normalized by dividing by the total count of mapped reads for the sample and multiplying by the maximum total read count across all samples.
This sample is included in the total count of ST20 and ST8 in this table.
In the present study there was a 39% reduction (P < 0.05) in the total virus count of samples stained at room temperature compared to samples heated at 80°C, which suggests that the high staining temperature is crucial to avoid underestimating the natural virioplanktonic abundance.
Inspired by [ 108] and also for computational efficiency, in this study we used N* defined as the total nucleotide counts minus the counts of the top 10% of genes with the highest counts to scale the gene expression levels in each sample, instead of using the total counts of mapped nucleotides in each sample.
For each sample, total counts of reads were normalized to 20 million.
Total counts of bacteria decreased in all flushed and nonflushed samples with time; however, flushed samples contained higher total counts of bacteria compared with those in nonflushed samples.
The total virus counts of samples passed through membrane filters with pore sizes of 0.22 μm and 0.1 μm were 7% and 24% lower than the counts of samples without filtration, respectively.
After removing redundant sequences and those with low read-counts (less than an average of ten total counts in all samples), the curated assembly consisted of 36,125 transcripts, with an N50 of 2.26 kb (Table 1).
