Sentence examples for total confluence from inspiring English sources

Wild-type HUVEC cells were grown in six-well microtiter plates to near total confluence in complete culture medium.

One day before scratching, the cells transfected with miR-143 inhibitor or NC were seeded into 6-well plates to almost total confluence in 24 hours.

The mitotic cycle of MSCs was synchronized at G1/G0 stages through 24 to 48 h contact inhibition of their proliferative activity after reaching the total confluence state under culture conditions in the medium enriched with 10% FBS.

A total of 1 × 10 cells were seeded onto a six-well plate and allowed to reach full confluence.

At a total cell confluence of 80%, chondrocyte differentiation was induced by the addition of ascorbic acid (50 µg/ml) and maintained throughout the experiment.

After approximately 3 weeks of culture and before reaching confluence, total RNA was extracted using High Pure RNA Isolation kit (Roche Diagnostics, Indianapolis, USA) according to manufacturer's instructions.

The contribution of the Presanella creek is 44% of the total flow at the confluence with the Vermigliana, while the contribution of the Presena creek rises to 75% of the total flow immediately after the confluence.

Ten percent of the US population, for example, accounts for 70percentt of total US health expenditures the confluence of the socioeconomically vulnerable and the medically frail.

The cells were grown to near confluence before total RNA extraction.

When the cells reached approximately 90% confluence (in total, approximately 1.8 × 10 cells), they were collected and washed three times with 1X PBS.

The observed lower level of total RNA in high confluence cells treated with 10 μg/ml prolactin, and potentially the inhibitory presence of CIS, may explain the reduction in signal observed in higher confluence cells at 10 μg/ml.