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Results For the microbiome (Table 6), NGS showed a total concordance for the detection of bacterial species (mainly Escherichia coli), in samples with single isolated bacteria.
Among the 148 intestinal samples from piglets with diarrhea, 136 and 118 were positive based on the TaqMan and conventional RT-PCR methods, respectively, indicating that the TaqMan RT-PCR was more sensitive than conventional RT-PCR, and the total concordance of the two methods was approximately 87.84%.
However, it is more likely that these artifacts would introduce inconsistencies between duplicated samples; this would result in an underestimation of the total concordance seen between duplicates in our study.
The total concordance between IHC and FISH was 95.6%.
The total concordance between IHC and FISH was 86.1% (Table 3).
Summary scores were calculated to measure the total concordance for screening, and for treatment.
Similar(43)
Inheritance checking of the commercial marker data identified that the results contained 0.13% human errors and 0.12% other errors (0.25% total error), whereas concordance checking found 0.16% human errors.
Repeated quality control genotypes (5% of the total) showed a concordance of 100.0%.
Repeated quality control genotypes (8% of the total) showed a concordance of 99.7%.
Total percentage of concordance for all pooled cases from different centers was 98.0% (range 94.4% to 99.4%).
A total number of 5559 concordance lines (an average of 80 concordance lines per consultation) were analysed by the first author in terms of the process type, the grammatical roles that patients see themselves playing and roles of other participants involved in the process.
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