Metabolically active cells in biofilms were around 17 35% of the total cells and 6 18% were able to form colony units in R2A medium.
Bacterial survival was assessed by culture, qPCR to quantify total cells, and propidium monoazide coupled with qPCR to quantify viable cells and highlight viable but non-culturable (VBNC) cells.
caused a significant reduction in the total cells and neutrophils in BALF, a significant reduction in the W/D ratio and EBA leakage at 24 h after LPS challenge.
A Labeling Index (LI), expressed as the number of BrdUrd BrdUrd-labeled cells per 100 total cells and the Numerical Density (ND), expressed as the total number of cells per 100 μm2 were calculated.
Results were expressed as percentage of TUNEL positive cells (over total cells) and normalized to control.
Similar to ICOS+/− mice, B7RP-1+/− mice had decreased total cells and eosinophils in the BAL than wild-type mice and significantly increased number of total cells and eosinophils than B7RP-1−/− mice (Fig. 5B).
The same field was used to identify total cells and cells expressing BMP-7 and phosphor- Smad 1,5,8.
After infection with invasive M90TδaroA shigellae, caspase-1 activation (Fig. 3A) was detectable in total cells and macrophage fractions of tumors taken 6 hours after infection.
We further detected two sub-populations of NCAM+ cells, NCAM+EpCAM− (13.5±4.9% of total cells) and NCAM+EpCAM+ (14.5±3.7% of total cells).
In addition, similar to CD24, triple FACS staining demonstrated a large population of CD133+EpCAM+NCAM− cells (29.5±10.6% of total cells) and a relatively small ones of the CD133+NCAM+EpCAM+ (14.4±4.5% of total cells) and CD133+NCAM+EpCAM− putative progenitor and stem phenotypes (1.1±1.2% of total cells) (Fig. 4b).
As expected, we found that the side population was made by a very small fraction (0.97%) of the total cells, and, interestingly, it was completely included within the CD133+ subset.
