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: total cells, A: AmCyan sorted cells, C: mCherry sorted cells.
b, Total cells sorted for each edited population.
Total cells were calculated as OD550 (adhesive cells + non-adhesive cells), and the percent adherence was calculated as 100 × OD550 (adhesive cells)/OD550 (total cells).
Black line: total cells, blue line: AmCyan cells, red line: mCherry cells.
Black line: total cells, blue line: AmCyan cells, red line: mCherry cells, and green line: mVenus.
(b) After staining, positively stained macrophages were counted and expressed as a percent of total cells.
(c) Representative fluorescence histograms of total cells stained with MitoTracker red CMXRos.
Black line: total cells, blue line: AmCyan cells, red line: mCherry cells and green line: mVenus.
Total cells, A: AmCyan sorted cells, and C: mCherry sorted cells.
Similar(2)
Results are expressed as F4/80 stained cells/total cells.
The percentage of dead cells was calculated as [ total cells - cells emitting fluorescence)/total cells] × 100.
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