Other studies however suggested that expression noise was rather a minor source of total cell-to-cell variations and showed that the differences may be caused by other factors, such as the capacity of individual cells to express proteins from genes (expression capacity).
We used the percentage of red-fluorescent cells in the total cells to represent the percentage of healthy cells with normal transmembrane potential (Δ ψm).
Comparing total cells to the time zero cell density revealed that 0.1 μM KU174 is as cytostatic as 10 μM 17-AAG.
Shortly thereafter, division of 3ab1, 3ab2, and 1abc122 bring the total cell count to 84.
There was also significantly reduced total cell recruitment to the airways (p<0.05, Figure 6b).
Subsequently, all three lavages were pooled and the cells were counted using a Bürker hemocytometer (total cell count) to assess airway inflammation.
The treatment of 3,4-DGE-damaged endothelial cells with citrate or CAG combination significantly reduced total cell death to 35%.
Percentage of viable cells was calculated based on the ratio of viable cell to total cell population in each well.
The relative contributions of lipid rafts from different subcellular compartments to the total cell lipid rafts remain to be determined.
The fused cells population counted 1.3±0.2% among total cells corresponding to 7.7±1.3% of DsRED positive cells.
