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The SVT016426-induced cell death inhibition was close to 20% of the total cell population.
Single cells represented approximately 30% of the total cell population, and cell clusters 70%.
Flow cytometry was performed on the total cell population (including both adherent and detached cells) and apoptosis detection was carried out as mentioned above.
The cell count of C. pasteurianum increased up to 90% of the total cell population when the system approached its maximum hydrogen production.
Astrocyte differentiation was only observed on <1 and 3.5 kPa surfaces and represented less than 2% of the total cell population.
Cresyl violet staining was performed to determine the total cell population.
Results were expressed as the percentage of apoptotic cells among the total cell population.
Quantitative results are expressed as a percentage of the total cell population (DAPI cell nuclei staining) (Fig. 6).
Expression of Sox10 and Olig1 was maintained in 34.01±9.67% and 53.79±13.29% of the total cell population, respectively.
The total cell population was collected and then stained with anti-CD3ε antibody and subjected to flow cytometry.
Both the CHX and ActD treatments induced almost 40% increase in the number of multinucleated cells in the total cell population.
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