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Total cell mass increases exponentially with time.
In the REG-80 cultures, only 10 mM of the glycerol substrate was consumed by C. necator H16 by 120 h pi, resulting in a total cell mass accumulation of 2.82 g/L (Fig. 2a).
The results of Vero cell growth kinetic study showed a ~10 fold increase of total cell mass in iCELLis Nano and packed bed bioreactor systems and the increase in cell mass obtained was comparable with roller bottle system.
Then, 2 mL of 0.025 М CdSO4 and 500 μL of 0.5 M Na2S water solutions (Sigma-Aldrich, USA, 98%% purity) were poured into a 100-mL flask with BY-2 cells with total cell mass of 613 mg.
However, the ratio of starch consumption per total cell mass between the first and last cycles was 1 to 0.77, showing that major of the displayed α-amylase retained activity after 50 h.
Similarly, only 7 mM of the glycerol substrate was consumed in the REG-GB cultures by 120 h pi, but the total cell mass accumulation was 3.99 g/L (Fig. 2a).
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When grown under anchorage-independent conditions in ultra-low attachment plates, the accumulation of cells for the various infected cell lines roughly paralleled the total cell masses seen in soft agar growth assays.
All copy numbers were calculated for the total growing cell mass by multiplying per nucleus copy numbers by total cell numbers.
From approximately 2×108 cells, we obtained some 500 μg of biotinylated proteins, representing ∼1 2% of the total cell protein mass.
Increased FDG uptake in tumors is generally correlated positively with the total tumor cell mass, and declines in FDG uptake with treatment are typically associated with response to therapy [10, 11].
It suggests that de novo proliferation of β cells accounts for the noted increase in total β cell mass.
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