This model distinguishes between the tumor cells' contributions from tumor-independent response to therapy and total plasma CAF correlating with antitumor activity.
The ratios of myofibroblasts among the total fibroblasts in CaF-29 and NF-29 cultures at the third passage were 42% and 18%, respectively.
Among the total population of CAFs, the percentage of SM-α-actin-positive cells and the intensity of SM-α-actin staining were significantly higher in HER2- and BLC-derived stroma (Fig 7Aa c; Fig 7Ad f) than in Lum-A (Fig 7Ag i).
In our previous study, we classified all stages of oral tongue SCC into CAF-poor, CAF-medium, and CAF-rich patterns for CAF density analysis (Table 1).
In total, nine primary cells CAF and NTF were subjected to adipogenic, osteogenic, and pancreatic in vitro differentiation in inducer medium.
In xenograft tumors generated by injection of wild-type mice with lung adenocarcinoma cells alone or in combination with CAFs, the total concentration of collagen cross-links was the same in tumors generated with or without CAFs, but coinjected tumors had higher hydroxylysine aldehyde-derived collagen cross-links (HLCC) and lower lysine-aldehyde-derived collagen cross-links (LCCs).
Fractionation confirmed significant reductions in filamentous insoluble levels of F-actin, SEPT2, and SEPT7 in Cdc42EP3-depleted CAFs; the total levels of actin, SEPT2, and SEPT7 were unaltered.
A total of 1 × 105 CAFs were seeded in 24-well plates and co-transfected with miR-200b/c mimics or miRNA control (miRNA NC), and pMIR-IKKβ (wild-type or mutant) or the control plasmid pRL-TK (Promega, Madison, WI, USA) using Lipofectamine 2000 (Invitrogen).
Total RNA was isolated from CAFs, AHFs, and NFs using the mir-VanaTM miRNA isolation Kit (Ambion, Austin, TX, USA) following the manufacturer's instructions.
