In total, five barley genetic maps [ 21- 25] were used to establish a barley neighbour map using the same criteria as the IBM neighbour map of maize [ 41].
Total yield, barley yield equivalent, land equivalent ratio (LER) and system productivity index (SPI) of mixtures exceeded those of sole crops especially when faba bean seed rate in the mixture was increased to 75 kg ha−1 (37.5%) or more.
The total number of barley WRKY genes will remain unknown until the sequencing of the barley genome is completed.
The simplest relationships are essentially total synteny between barley 3H versus rice 1 (3HS = 1S, 3HL = 1L) and barley 6H versus rice 2 (6HS = 2S, 6HL = 2L).
The total number of barley genes was estimated to be around 30,400, and 26,159 (86%) of them are reported as high-confidence (HC) genes [ 23].
We have generated 574 Mbp of Illumina/Solexa sequences from barley total genomic DNA, representing about 10% of a genome equivalent.
Crop density (percent of county area that was harvested) was estimated for total agricultural land, barley, dry beans, corn, hay, oats, sorghum, soybeans, sugar beets, and wheat.
A total of 12 barley genes was subsequently analysed for transcript levels using QPCR, as described by Burton et al. [ 31].
Templates of 5 μg total RNA for barley and 0.5 μg total RNA for wheat were used for the cDNA synthesis reaction with Superscript III RNAse H-Reverse Transcriptase (Invitrogen, Australia) according to the manufacturer's protocol.
Briefly, poly(A) RNA was extracted from total RNA of barley plants using the Oligotex kit (Qiagen) and then ligated with a 5′ RNA adaptor containing a MmeI restriction site using T4 RNA ligase (Invitrogen), followed by reverse transcription, second-strand synthesis, MmeI digestion, ligation of a 3′ dsDNA adaptor, gel-purification, and PCR amplification.
