Exact(4)
After 30 minutes of incubation, approximately 17.4% of the total attached spores were seen surrounded by enriched F-actin staining (SEM = 1.9%, n = 6 independent assays) (Fig. 1, D – G), suggesting that there was local activation of actin polymerization or reorganization at these sites.
In fact, after 1 day, total attached HUVECs numbered less than those on unmodified grafts, and after 3 days, total metabolic activity of HUVECs was decreased compared to controls.
Moreover, the percentage of membrane-damaged cells attached on the glass surface after incubation for two hours increased more than 2-fold in the presence of dATP treatment (9.58% with dATP treatment vs. 3.99% without dATP treatment; p value <0.01) and total attached cells increased from 3.02×105 cells/cm2 to 7.97×105 cells/cm2 (Figure 5).
The number of total attached cells was evaluated by DAPI staining.
Similar(56)
Attachment percentage was calculated by comparison with the total cell numbers seeded on grafts, which was normalized to total cells attached to separate tissue culture polystyrene (TCPS) controls.
"I turned down $5m from a very important lobbyist because there are total strings attached to a thing like that.
"Finally, on the third follow-up (and fourth email total), I attached the damn thing," recalls Nathan.
Nor should the 7,696 total be attached to the base $1.448 billion "project," since the arena's already funded.
Six subsamples of water were filtered from each site; three were filtered using 0.2-μm-pore-size filters (30 ml, cellulose acetate membrane filter; Sartorius), and three were filtered using 3.0-μm-pore-size filters (cellulose nitrate membrane filter; Sartorius) in an attempt to estimate total and attached bacterial loads, respectively (Fig. 1) (24, 24).
In Fig. 3, the release is expressed in wt% of the total amount of MDS attached to the nanoparticles calculated using an UV-Vis absorption calibration curve.
After exposure to peristaltic flow for 18 h, there was a qualitative decrease in the total number of attached cells, but this difference was not significant compared to the degree of attachment before flow (P = 0.086, Fig. 9a).
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