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Without PCR the total assay time amounts to 27 min.
Total assay time is typically 60 minutes to 90 minutes.
The total assay from seawater sampling to data acquisition can be performed within a working day.
The total assay time is 5 min per sample, including the regeneration step.
Total assay time is about 1 h (but in the presence of hypofibrinolysis it can be prolonged).
Using the combined RT-LAMP and LFD system, the total assay interval was approximately 70 min, excluding RNA extraction time.
An aliquot of 25 μl of 2 mg/ml fluorescamine was added to a total assay volume of 100-μl diluted liposome suspension.
The total assay mixture consisted of 1.0 mL lipase solution, 2.5 mL olive oil emulsion and 0.02 mL of 20 mM CaCl2 · 2H2O.
With its proven performance as a "total assay," the CUPRAC reagent is an attractive candidate as a derivatization reagent in an HPLC post-column instrumental setup.
Thus, 10 min for rapid DNA extraction followed by LAMP combined with LFD detection resulted in a total assay time of approximately 50 min.
The HGIMS system had higher detection sensitivity using than the conventional method, and the total assay time, including sample preparation, was about 3.5 h.
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