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This was also supported by our mathematical models for tumor growth to compare the total and viable tumor burden in our model.
Total and viable bacteria counts decreased for the first 2 h after feeding then increased 4 h post-feeding.
The parameters used to estimate cell damage were the total and viable cell count of the population, the proliferating ability and the cytoskeletal structure of the treated cells.
However, only B indicus heifers showed a variation in the number of visualized follicles and the total and viable oocytes along consecutive OPU sessions.
Total and viable cell numbers were measured using a Vi-cell XR cell viability analyzer from Beckman Coulter Canada Inc. (Mississauga, ON, Canada).
Aliquots were counted for total and viable cell numbers on the specified days and fresh media was added to replenish and maintain the total volume of media in the flask.
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I. Total microscopic and viable counts and counts of specific organisms.
The cells were stained with 0.2% trypan blue dye, and the total cells and viable cells were counted under a light microscope.
The total cells and viable cells (cells that excluded blue dye) were counted using a hemocytometer (Bürker-Türk, Hirschmann Laborgeräte GmbH & Co. KG, Eberstadt, Germany) under a light microscope.
To understand this mechanistically, we developed a simple mathematical model to explain the observed tumor growth data of total tumor volume and viable tumor volume with blood Gluc value (Fig. 3D, Table S2).
Trypan blue staining was used to determine the total cell counts and viable cells on days 1 (24 h), 3 (72 h), and 5 (120 h) after siRNA transfection or irradiation.
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