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Sorted cells were used for the isolation of total and small RNAs.
An analysis of the microcirculation (Table 3) should be reported for both total and small (<20 μm) vessels.
Total and small RNA from PER, PSU, and RNO fibroblast cell lines was isolated using mirVana Isolation Kit (Ambion, Invitrogen Life technologies), following manufacturer's recommendations.
Besides, the fold changes in the expression levels of total and small RNA in each species were calculated using total RNA as control.
The levels of PSUcentSat transcription in both total and small RNA is higher in PER and lower in RNO, relatively to what happens in PSU (fig. 5 A and B).
Interestingly, we have demonstrated the presence of PSUcentSat transcripts in the total and small RNA fraction of these species' cells, being the level of transcription significantly higher in PER in comparison to PSU.
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The Polynesian minority comprises Wallis and Futuna islanders, who make up about one-tenth of the total, and smaller numbers of Tahitians.
A longer period of grazing had a positive effect on total and small-scale species diversity in both islets and fields.
It has been found that a relatively small total width and small rib-to-total width ratio are better for generating high power density in the fuel cell.
Significant correlations were found in women population and in postmenopausal women between Ox-LDL and total, large, and small HDL-c and between TNF- α and total, large, and small HDL-c, LDL-c, and Ox-LDL.
To test the method of RNA preparation in this study, total RNA and small RNAs were extracted with two different reagents.
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