In selecting an RNA isolation kit, not only must the ability of the kit to recover the required amount of total RNA be taken into consideration, but also the ability of the kit to recover RNA meeting specific quality criteria.
Briefly, equal amounts of total RNA were taken from each replicate on individual CCs of each patient group, denatured and reversed transcribed using the SensiScript reverse transcriptase kit (Qiagen, Mississauga, ON, Canada).
Furthermore vitamin D can be taken daily or the total amount can be taken once a week or even once a month as long as the total is the same i.e., 3000 IUs daily or 21 000 IUs weekly or 90 000 IUs monthly are equally effective in maintaining serum 25(OH D levels in the desired range of 40 60 ng/mL.
The total amount of blood taken during this study was approximately 28 ml from the 5 to 10 years old and 44 ml from the 11 to 14 years old children.
These men thought up the idea as a means of benefiting the hospitals, and, in exchange for government sanction, put up the capital to guarantee the first sweepstake, stipulating that they would require a certain percentage of the total amount of money taken in to pay for the cost of running it, and to pay themselves for their time and trouble.
Prospectors receive a royalty on the total amount of gold taken from a mine.
Of the total amount of space taken by law firms, 1.1 million square feet was expansion, helping to stabilize the market, said Lisa J. Kiell, executive vice president of Jones Lang LaSalle.
It is 25 square feet — not 1,500 square feet, the total amount of space taken up by all gardens at the building.
David Cameron promised "radical reform" of the system, but a Treasury review has only pledged to look at the way the system is administered, not the total amount of tax taken.
In all, the total amount of income taken home by American households will still probably rise next year, because the population will grow and government transfer payments (like jobless benefits) will surely increase.
