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Quantification of MCETs in a blinded fashion revealed that extracellular trap formation increased over time being significantly different from uninfected controls, but rarely exceeded 5% of the total amount of cells (Fig. 4A).
Amount of live cells (qPCR-PMA) was subtracted from the total amount of cells (qPCR) to obtain the number of dead cells (Δlog10 cells).
The total amount of cells present in the wells after simulated ischemia was counted using a Coulter Counter (Beckman).
The total amount of cells was determined by counting the number of nuclei using DAPI (Boehringer Mannheim).
This relation was used to calculate the percentage of cells within the biofilm related to the total amount of cells in biofilm and planktonic cells (see Fig. S2 and Table S1).
The total amount of cells used for sorting was adjusted to ensure that more than 1×105 cells could be harvested, which were then collected in 5 mL of SDCAA medium and grown overnight at 30°C with shaking.
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Table 2 shows the evolution of the total amount of cell-associated DNA from HIV.
The total amount of cell surface I-PFO* binding was determined.
After 2 hr at 4°C, the total amount of cell surface binding of I-PFO* was determined.
Cell number was assessed indirectly by staining with crystal violet dye, since the total amount of cell-bound dye is proportional to cell number (Lee et al, 2001).
Carbohydrate concentration measurements – glucan, xylan, NDF – relate to total cell wall concentration and thus the total amount of cell wall carbohydrates that could be converted into ethanol.
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