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This ssDNA was then converted into double stranded DNA using the WT-Ovation™ Exon module, originally designed for cDNA library synthesis, resulting in concentrations ranging between 300 500 ng/µl with the total amount obtained ranging from 9 to 15 µg.
This RNA was then converted into large quantities of single stranded DNA through the use of NuGEN WT-Ovation™ Pico RNA Amplification System, originally designed to process samples for microarray analysis, which yielded between 150 350 ng/µl of DNA with the total amount obtained ranging from 4.5 to 10.5 µg.
The total amount obtained from one reaction batch was 5.3 mg, corresponding to an isolated yield of 46% (compared to the end of biocatalytic synthesis).
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The 10 samples with suboptimal quality control criteria were characterized by either a low RNA integrity number suggestive of RNA degradation (n = 2), a low cRNA amount obtained after total RNA amplification and labeling (cRNA hybridized on BeadChips < 750 micrograms) (n = 7) or both (n = 1) (Tables 2 and 3).
Deduct total liabilities from total assets to obtain the residual amount or the Equity value.
A small increase in the amount of total carotenoids was obtained by overexpressing the gene encoding phytoene synthase-lycopene cyclase (crtYB) by integrating multiple copies of this gene into the ribosomal DNA [ 17].
The amount of collagen per 10 μg total protein was obtained from the standard curve and multiplied by the total protein to give total water soluble collagen levels.
The International Labour Organization ILOO) estimates that the total illegal profits obtained from the use of forced labour worldwide amount to over $150 billion per year.
To evaluate protein recovery, we compared the total amount of protein obtained with modified TRIzol protocol, with that obtained using commercial (mirVana™ PARIS™), standardized laboratory (A+2X buffers), and TRIzol® total protein extraction methods.
This study showed a same phenomenon that a linear relationship was found between the charge passed during the RCPT and the total amount of chloride obtained from the chloride profile.
However, the total amount of hydrogen obtained in that study (Yokoyama et al. 2003a) was the average value of the entire specimen.
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CEO of Professional Science Editing for Scientists @ prosciediting.com