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Because short AARs may be derived from the interruption of a long AAR, we used repeated residues per 1000 amino acids (RRPK, Repeated Residues per Kilo Amino Acids, defined as the ratio of the total AAR length to the protein length, multiplied by 1000) to represent the AAR content of a protein or protein segment.
Myocardial infarct size was determinated as a percent of infarct zone (INF) over total AAR (INF/AAR × 100%%).
The data screening tests described in Sections 2.4.1 3, with the application of a 2.5 stdev cut-off value, resulted in the exclusion of 28 data points from the total AAR data set (in which analytical replicates were counted as individual data-points) of >500 (5%).
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Among the AAR, three AAR accounted for 50% of the total: Glutamine (Gln), 174 repeats, 29%; Serine (Ser), 75 repeats, 12.9%; and Glycine (Gly), 66 repeats, 11.1%.
The weighting is based on the length of the coding sequence of each individual repeat, as described in the following equation; (2) where n is the total number of AARs on the protein-coding gene, l is the length in bp of each individual repeat, P is the corresponding Purity and L the sum in bp of the length of all AARs in the gene.
†Significantly (P < 0.05) different from CON. LV weight, AAR weight, and the ratio of AAR to total LV mass were similar among the groups (Table 3).
Including these molecular function terms in the linear model to explain the number of AARs per gene, the total percentage of variance explained by significantly enriched GO terms is 13.9% for human and 15.2% for mouse (see Table 7 for human and Table S9 for mouse).
The LV area, AAR, the area of infarction of each slide and the total volume of the damaged myocardium were then determined as previously described (Masini et al., 2002).
Immediately after the AAR meeting breaks up, commanders gather their units to conduct their own AARs.
AAR was also examined in both the Free Amino Acid (FAA) and corresponding Total Hydrolysable Amino Acid (THAA) fractions.
All values were taken directly from the CAPTE AAR with the exception of programmatic diversity which was calculated by taking the total of non-white students divided by the total cohort, multiplied by 100.
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