Fifty-one DIPG patients had sufficient biological material to perform targeted sequencing of H3F3A, HIST1H3B/C, PIK3CA, TP53 and ACVR1 using Fluidigm array and ion torrent chips (Life Technologies).
And last month, researchers in Germany and China reported that in just 3 days, they had independently used Ion Torrent's chip readers to decode the Escherichia coli strain that recently killed more than 30 people in Europe.
An Ion Torrent 314 chip has been used following the manufacturer instructions (Life Technologies, Carlsbad, USA).
All libraries were PCR amplified and sequenced using an Ion Torrent 318 chip as per manufacturer's instructions, with the difference in library size in the CircLig protocol accounted for to ensure the same molarity of each library was used.
To increase the throughput, the Ion Torrent sequencing chip makes use of a highly dense microwell array in which each well acts as an individual DNA polymerization reaction chamber.
The desired size fragments were ligated with the library adaptors and subjected to emulsion PCR, followed by its recovery and loading onto Ion Torrent PGM 316 chip.
The purified Sf101 phage DNA was sequenced using the Ion Torrent PGM 314 chip (Life Technologies) at the Australian Genome Resource Facility, University of Queensland.
Before sequencing, small aliquots of libraries were pooled and sequenced on an Ion Torrent PGM 314 chip after additional amplification with PGM fusion primers.
Additionally, it offers higher quality than 454, especially when sequencing homopolymers, but at a similar cost (about US$23 per Mb for the Ion Torrent PGM −314 Chip).
The Ion Torrent machine uses chips like those in a computer to do sequencing, rather than the complicated cameras and lasers most other sequencers use.
Sequencing was performed with Ion Torrent PGM 316 chips.
