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Since stem cells stand at the top of cell lineages, they can help minimize this accumulation by maintaining a high-quality genome and periodically refreshing a pool of cells that accumulate mutations at a higher rate but that are only transiently present in the tissue.
For instance, a recent study identified factors involved in the transition between cell shapes using automated phenotyping of human cell cultures that took advantage of fluorescent staining for DNA, tubulin and actin on top of cell morphology (Fuchs et al., 2010).
Subsequently, 30 μl of fixative solution (4% paraformaldehyde, 3.4% sucrose), 60 μl of 1% lipsol and 60 μl of fixative solution were added on top of cell suspension and spread using a glass rod seven to ten times back and forth.
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Embryo development occurred on top of cell-cultured scaffold, which suggests that it is not embryotoxic.
Macrophages were seeded on top of cell-laden hydrogels to mimic in vivo macrophage interrogation and treated with lipopolysaccharide to induce an inflammatory phenotype.
In Excel and PowerPoint, you can also draw on top of cells and slides, respectively.
To perform an activity such as a movement, the agents on top of cells utilize the spatial information to recognize the morphological features of the space underneath them (which include streets, obstacles and displays).
One day after cell seeding, 1 ml of culture media containing PA along control (1 µg/ml PA) or LeTx (1 µg/ml PA and 0.01 µg/ml LF) were added on the top of cell-containing agar layer.
An anisotropic cell sheet placed on top of other cell sheets in fabricating thick tissue was able to change the cell orientation in several layered cell sheets underneath.
Notably, in this situation, DE-cadherin serves as the substrate that promotes the migration of cells on top of other cells [ 14].
This thirteen country report found what every study that has ever examined people who have used phones for a decades or more has determined top users of cell phones had a doubled risk of malignant tumors of the brain.
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Since I tried Ludwig back in 2017, I have been constantly using it in both editing and translation. Ever since, I suggest it to my translators at ProSciEditing.

Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com