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I think he's on to something.Mr Murray envisions a chap named Hank who builds a chain of auto-repair shops that make him very rich, but not particularly powerful:[Hank] is not just in the 1%; he's in the top fraction of the 1% but he's not part of the new upper class.
Finally, we calculate ACT-50 %, which corresponds to the top fraction of the ranked library that contains half of the active compounds.
Effect of pH on recovery, PF, KE, and VR of alkaline protease in the PEG-rich top phase compared to those values of the top fraction of previous step was depicted in Figure 2.
To increase the protease recovery and purity, additional sodium citrate 10, 15 and 20% (w/w) was added to the first cycle top fraction from 70% (w/w) CE with 15%PEG2000-15PEG2000-15%itrATPSATPS.
These include enrichment factors calculated for the top 1 and 10%% of the ranked library (EF1 % and EF10%%), the Boltzmann-Enhanced Discrimination of Receiver Operating Characteristics (BEDROC20) score, the area under the enrichment curve (AUC), and the top fraction of the ranked library that contains 50%% of the active compounds (ACT-50ACT-50%
Ranking accuracy using the default and optimized box size is evaluated by the enrichment factor for the top (a) 1 % and (b) 10%% of the ranked library, (c) Boltzmann-Enhanced Discrimination of Receiver Operating Characteristics, (d) the area under the enrichment curve, and (e) the top fraction of the ranked library that contains 50%% of actives.
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Cytoplasmic extracts were fractionated through sucrose gradients, with the lightest components appearing at the top (fractions 1 and 2), small (40S) and large (60S) ribosomal subunits, and monosomes (80S) in fractions 3 6, and progressively larger polysomes in fractions 7 12.
After centrifugation, 3 ml of top fractions were mixed with 0.5 ml of biuret reagent [9] and used for the measurement of optical density (λ = 550 nm) on "Shimadzu UV-1800" spectrophotometer.
Rn, Re and Rt represent the coverage rates of proteins in the nucleoid, envelope, and top fractions, respectively.
DPPE was recovered mostly in the bottom fractions (over 80% of DPPE was present in fractions 8 10), whereas majority of SM or SLPE was retrieved in the top fractions (fractions 1 3).
The csNAPs were selected as those proteins that were relatively abundant in the nucleoid fraction as compared with in the envelope and top fractions.
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