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They demonstrated that with most methods, over 90% of differently expressed genes at the top expression levels could be detected with using two replicates and 5% of the reads [ 30].
AGP related DEGs were downregulated in FLA17 and upregulated as was seen in FLA1 (top expression in yellow stage).
Since some protein sequences are 5′ truncated or predicted starting from an upstream point, we manually checked the prediction of top expression of transcripts containing signal peptides.
(B) Top: Expression of p-EGFR, p-AKT, α-SMA, cyclin D1, and p-Smad in sectioned rat liver was examined by immunohistochemistry.
The top expression quartile of genes from [41] harbors somewhat wider peaks than the lowest quartile (means: 176 vs. 172 bp, respectively, p = 0.002; Figure 8).
In fact, all RP genes in E. coli do not reach the top expression level.
Similar(47)
We classified genes as either expressed (top 90% expression quantiles) or silenced (lowest 10% quantile) and as either methylated (top 90% methylation quantiles) or unmethylated (lowest 10% methylation quantile) to generate contingency tables for each component of the gene cassette.
Rogen, of course, is a maximalist, an engine of over-the-top expressions of voracious desires (the comic drama lies in taming these impulses).
When you express guilt about indulging in sweets, in anger, in infidelity, you are directing attention to the negative effects that will occur in some future time, e.g., gain of weight, pimples, diabetes, reprisal for over-the-top expressions of emotion, or for being caught in flagrante.
Over-the-top expressions of feelings for you even though you haven't met are a warning sign.
Gametocyte-stage specific expressed genes were defined as all genes among the top 30% expression levels at the gametocyte stage and the bottom 50% expression levels in all other stages.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com