Sentence examples for top down experiments from inspiring English sources

Several groups have applied label-free quantitation to comparative top down experiments on a few, targeted proteoforms.

The high mass accuracy product ion measurements achieved in top down experiments provide an impressive level of specificity, requiring relatively few matching fragments for a positive identification.

Ribosomal proteins constitute a large proportion of the proteins identified in top down experiments due to both their high abundance relative to the basal proteome during normal growth conditions and high ionization efficiency in positive polarity (stemming from the high average isoelectric point).

Although internal ion formation can be used for diagnostic purposes in top down experiments with extensive a priori knowledge of the protein of interest, accommodating internal ions in a high throughput identification search strategy would cause a prohibitively large increase in fragment ion search space.

Unfortunately top-down experiments on this protein did not produce reliable sequence information.

MS/MS top-down experiments were also run in an effort to identify the observed proteins.

However, the high complexity of the antennal extract mixtures and the low concentration of single proteins in the extract limit the application of top-down experiments.

To identify the modification site(s), the precursor ion corresponding to the modified protein (m/z 1415.9 Da, 10+ charge state) was isolated for top-down experiments using μESI-FTICR-MS with ECD (Figure 1B, upper panel).

Although the LTQ-Orbitrap mass spectrometer is designed for MS/MS experiments on peptides and small proteins, previous top-down experiments on isolated and abundant proteins belonging to the OBP family showed that spectra did allow obtaining short amino acid sequences.

The identification of the protein as OBP-9 was also supported by the measure of the monoisotopic mass (13,926.71 Da fig. S2 D, the theoretical monoisotopic masses of OBP9 and OBP6 being respectively 13,926.77 and 13,925.80 Da respectively) and by the top-down experiments, which produced a spectrum compatible with the N terminal EFVV stretch of OBP9 (see supplemental material Text S1 and fig. S2 E).

We demonstrate the use of NeuCode SILAC to enable multiplexed-quantitation for top-down experiments.