This stock solution was diluted (1 100) in a diluent containing 1% acetone and 0.02% Triton X-100 (Sigma) to obtain the top concentration of 12 µM.
For single cycle kinetic analysis, HAdV-D26K, HAdV-D48K, HAdV-B35K, HAdV-C5K, and HAdV-B3K were injected at a top concentration of around 200 µM, followed by four injections using serial 1 3 dilutions.
Eight two-fold serial dilutions were performed with a top concentration of 150 μM.
In seam-XI bottom, concentration of mineral matter range from 2.30%% to 22.40 %, mean 9.74%% and in seam-XI top, concentration of mineral matter range from 10.20 % to 51.70 %, mean 15.56 %.
Each plate was then coated with eight two-fold serial dilutions of human IgG reference standard at a top concentration of 0.625 μg/ml.
Each chemical was run in 8 serial dilutions using half-log spacing descending from a top concentration of 50 0.023 μM (or 20 μM–0.009 μM for CYP assays).
MCF-7 cells were cultured either in the presence of increasing concentrations of fulvestrant (top concentration of 100 nM) (FulvR1a, FulvR1c, and FulvR2a) or in the absence of estrogen (FulvC1a, FulvC1b, and FulvC2).
Injections of nanodiscs over G protein showed some concentration-dependence in the signal at higher concentrations, which was partially abolished by lowering the top concentration of analyte (Fig. 2A and B).
HPLC fractions 5 and 6 were tested at a top concentration of 1 40 and also in a 3-fold dilution series of this concentration, whereas the remaining fractions were tested only at a 1 40 final dilution of the sample.
To determine the effect of the mGlu5 PAMs on the glutamate dose−response relationship, cells received the test compound (12.5 μM) or vehicle followed by increasing concentrations of glutamate (with a top concentration of 100 μM).
Inhibitor solutions were prepared from solid stocks and dissolved in assay buffer and a concentration series of 3-fold dilutions from a top concentration of 100 μM was created.
